Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free (ab224262)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase - Low endotoxin, Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free
    See all Indoleamine 2, 3-dioxygenase primary antibodies

  • Description

    Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate,

  • General notes

    ab224262 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free (ab224262)
    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free (ab224262)
    Lane 1 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
    Lane 2 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa



    The expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.

  • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free (ab224262)
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] - Low endotoxin, Azide free (ab224262)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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