Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP) - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free
    See all Placental alkaline phosphatase (PLAP) primary antibodies

  • Description

    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant full length protein within Human Placental alkaline phosphatase (PLAP). The exact sequence is proprietary.

  • Epitope

    Not determined

  • Positive control

    • IHC-P: Seminoma and placenta tissues. Flow Cyt: HeLa cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab218848 is the carrier-free version of ab16695 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab218848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    SP15

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)
    Flow Cytometry - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA and sodium azide (ab16695).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)

    Human seminoma stained with ab16695.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA and sodium azide (ab16695).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)

    Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab16695 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA and sodium azide (ab16695).

  • Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)
    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] - BSA and Azide free (ab218848)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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