Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRBOB-2-F1-17] to Apolipoprotein L1/APOL1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free
    See all Apolipoprotein L1/APOL1 primary antibodies

  • Description

    Rabbit monoclonal [EPRBOB-2-F1-17] to Apolipoprotein L1/APOL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HDLM-2 whole cell lysates; human liver lysate; human plasma. IHC-P: Human colon and kidney tissue. IP: HepG2 whole cell lysate.

  • General notes

    ab252392 is the carrier-free version of ab252218.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Apolipoprotein L 1/APOL1 with ab252218 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on glomerulus and weak cytoplasmic staining on renal tubules (PMID: 21997392, PMID: 28724794, PMID: 27138898) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252218).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Apolipoprotein L 1/APOL1 with ab252218 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining mainly in plasma of human colon blood vessels is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252218).

  • Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)
    Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)

    Apolipoprotein L 1/APOL1 was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab252218 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252218 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (Input).
    Lane 2: ab252218 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252218 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252218).

  • Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)
    Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-17] - BSA and Azide free (ab252392)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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