Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
![Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.com/ps/products/108/ab108315/Images/ab108315-120870-anti-apolipoprotein-l-1-antibody-epr29072-western-blot.jpg "Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)]
See all Apolipoprotein L1/APOL1 primary antibodies -
Description
Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma, ovary cancer, and HepG2 cell lysates. ICC/IF: BxPC-3 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Apolipoprotein L 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2907(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)All lanes : Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.com/ps/products/108/ab108315/Images/ab108315-4-anti-apolipoprotein-l1-apol1-antibody-epr29072-immunocytochemistry-bxpc3-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labeling Apolipoprotein L1/APOL1 with purified ab108315 at 1/50 dilution (9.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Flow Cytometry - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.com/ps/products/108/ab108315/Images/ab108315-257551-anti-apolipoprotein-l-1-antibody-epr29072-flow-cytometry.jpg)
Flow Cytometry - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.com/ps/products/108/ab108315/Images/ab108315-257552-anti-apolipoprotein-l-1-antibody-epr29072-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.
Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.
Lane 1:Human plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.com/ps/products/108/ab108315/Images/ab108315-5-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
