Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)]
    See all Apolipoprotein L1/APOL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human plasma, ovary cancer, and HepG2 cell lysates. ICC/IF: BxPC-3 cells.

  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Apolipoprotein L 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    All lanes : Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human ovary cancer lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labeling Apolipoprotein L1/APOL1 with purified ab108315 at 1/50 dilution (9.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    Flow Cytometry - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2(Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

    Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

    Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

    Lane 1:Human plasma

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
    Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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