Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calpain 1 antibody [EPR3319] (ab108400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3319] to Calpain 1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calpain 1 antibody [EPR3319]
    See all Calpain 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3319] to Calpain 1

  • Host species

    Rabbit

  • Specificity

    ab108400 recognises the large subunit of Calpain

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, U937, A431, (D) 293T, and T47D cell lysates; Human kidney tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400)

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : CAPN1 (Calpain 1) knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate

    Predicted band size: 82 kDa



    Ab108400 was shown to recognize Calpain 1 in wild-type HAP1. A faint cross-reactive band was observed in  CAPN1 (Calpain 1) knockout cells at 82 kDa when over-exposed. Due to the immunogen’s homology with CAPN2 (Calpain 2 Uniprot: P17655), this band could correspond to the Calpain 2 protein. Please note that cross-reactivity with this protein has not been confirmed experimentally.

  • Flow Cytometry - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Flow Cytometry - Anti-Calpain 1 antibody [EPR3319] (ab108400)

    Overlay histogram showing Jurkat cells stained with ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (ab108400)

    ab108400 staining Calpain 1 in the T-47D cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/70). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)

    ab108400 staining Calpain 1 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/200). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution + C6 Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + T47-D Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa

  • Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Western blot - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal kidney cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : T47D cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 1 antibody [EPR3319] (ab108400)

    ab108400, unpurified, at 1/500 dilution staining Calpain in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (ab108400)

    ab108400, unpurified, at 1/250 dilution staining Calpain in HeLa cells by Immunofluorescence.

  • Anti-Calpain 1 antibody [EPR3319] (ab108400)
    Anti-Calpain 1 antibody [EPR3319] (ab108400)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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