Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)
![Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)](https://www.abcam.com/ps/products/252/ab252219/Images/ab252219-4-252219IHC2.jpg "Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRBOB-2-F1-53] to Apolipoprotein L1/APOL1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53]
See all Apolipoprotein L1/APOL1 primary antibodies -
Description
Rabbit monoclonal [EPRBOB-2-F1-53] to Apolipoprotein L1/APOL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and HDLM-2 whole cell lysates; human plasma; human liver lysate. IHC-P: Human colon and kidney tissue. IP: HepG2 whole cell lysate.
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General notes
This product was previously labelled as Apolipoprotein L 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPRBOB-2-F1-53 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Apolipoprotein L 1/APOL1 with ab252219 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on glomerulus and weak cytoplasmic staining on renal tubules (PMID: 21997392, PMID: 28724794, PMID: 27138898) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)](https://www.abcam.com/ps/products/252/ab252219/Images/ab252219-3-252219IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Apolipoprotein L 1/APOL1 with ab252219 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining mainly in plasma of human colon blood vessel is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)](https://www.abcam.com/ps/products/252/ab252219/Images/ab252219-2-252219IP1.jpg)
Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)Apolipoprotein L 1/APOL1 was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab252219 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252219 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (Input).
Lane 2: ab252219 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252219 in HepG2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds. -
![Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)](https://www.abcam.com/ps/products/252/ab252219/Images/ab252219-1-252219WB1.jpg)
Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)All lanes : Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Human plasma lysate
Lane 3 : HDLM-2 (human Hodgkin lymphoma) whole cell lysate
Lane 4 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDaExposure times:
Lane 1: 37 seconds
Lane 2: 5.5 seconds
Lane 3 and 4: 81 secondsThe expression profile/ molecular weight observed is consistent with what has been described in the literature and the doublet band in lane 2 might be the different isoform of ApoL1 (PMID: 25012173, 23300552).
Blocking/diluting buffer and concentration: Blocking buffer and concentration
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![Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)](https://www.abcam.com/ps/products/252/ab252219/Images/ab252219-5-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein L1/APOL1 antibody [EPRBOB-2-F1-53] (ab252219)
