Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein
Suitable for: IHC-P, WB, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310]
See all Myelin oligodendrocyte glycoprotein primary antibodies
Description

Rabbit monoclonal [EPR22629-310] to Myelin oligodendrocyte glycoprotein
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain tissue lysate; mouse brain tissue lysate; rat brain tissue lysate. IP: Mouse brain tissue lysate. IHC-P: Human cerebellum tissue; mouse cerebellum tissue; rat cerebellum tissue. IHC-Fr: Mouse cerebellum tissue; rat cerebellum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22629-310
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in rat cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 26 seconds; Lane 2: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of rat cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Myelin oligodendrocyte glycoprotein was immunoprecipitated from 0.35 mg of mouse brain tissue lysate whole cell lysate with ab233549 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233549 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10 μg (Input).
Lane 2: ab233549 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233549 in mouse brain tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549) at 1/1000 dilution + Human brain tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution (green). Positive staining on nerve fiber tracts in mouse cerebellum (PMID: 26347141) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of mouse cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling Myelin oligodendrocyte glycoprotein with ab233549 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on white matter of human cerebellum (PMID: 25421634) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab233549 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] (ab233549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com