Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

500 µl 298 185 ₸ 1 ml 469 056 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
Suitable for: Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]
See all Placental alkaline phosphatase (PLAP) primary antibodies
Description

Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

IHC-P
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is considered to be commercially sensitive.
Epitope

Not determined
Positive control
- IHC-P: Seminoma and placenta tissues. Flow Cyt: HeLa cells
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

This product was switched from a hybridoma to recombinant production method on 07^th March 2019.

This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Primary antibody notes

This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
Clonality

Monoclonal
Clone number

SP15
Isotype

IgG
Research areas

Flow Cytometry - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

This image was generated from the hybridoma version.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Human seminoma stained with ab16695.

This image was generated from the hybridoma version.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.

This image was generated from the hybridoma version.
Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com