Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4435(2)] to ACE2 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free
    See all ACE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4435(2)] to ACE2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Human, Synthetic fragment
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab198988)

  • Positive control

    • IHC-P: Human, mouse, and rat kidney tissues; WB: Caco-2, HepG2 and Calu-3 cell lysates; Human fetal kidney and human testis lysates; IP: Human testis tissue lysate.

  • General notes

    ab239924 is the carrier-free version of ab108252.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Wild-type HepG2 cell lysate
    Lane 2 : ACE2 knockout HepG2 cell lysate
    Lane 3 : Calu-3 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab108252).

    Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108252 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line ab273733 (knockout cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)

    ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded human kidney tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108252).

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Wild-type Caco-2 cell lysate
    Lane 2 : ACE2 knockout Caco-2 cell lysate
    Lane 3 : Calu-3 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab108252).

    Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108252 was shown to react with ACE2 in Caco-2 wild-type cells in western blot with loss of signal observed in ACE2 knockout cell line ab273731 (knockout cell lysate ab275516). Wild-type and ACE2 knockout Caco-2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)

    ab239924 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 2 μg primary antibody (1/50). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.

    Exposure time: 1 second.
    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.



    All lanes : Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924) at 1/500 dilution

    Lane 1 : Human testis tissue lysate at 10 µg
    Lane 2 : ab239924 + Human testis tissue lysate
    Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab239924 in Human testis tissue lysate

    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)

    ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded human testis tissue by immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108252).

  • Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
    Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)

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