Anti-ACE2 antibody (ab87436)
Key features and details
- Rabbit polyclonal to ACE2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cat, Dog, Orangutan
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Immunogen
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Positive control
- WB: Human kidney tissue lysate (ab30203). IHC-P: Mouse heart tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). Application notesIs unsuitable for ICC/IF.Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Western blot - Anti-ACE2 antibody (ab87436)Anti-ACE2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 115 kDa (possible glycosylated form)
Exposure time: 30 secondsACE2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
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Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436)This image is courtesy of an anonymous Abreview.ab87436 staining ACE2 in mouse heart tissue sections by immunohistochemistry. Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with ab87436 (1/100 dilution) for 18 hours at 4oC. A HRP-conjugated goat anti-rabbit polyclonal (1/50) was used as the secondary antibody.
Protocols
Datasheets and documents
References (7)
ab87436 has been referenced in 7 publications.
- Joshi S et al. Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34+ cells. J Cell Physiol N/A:N/A (2019). PubMed: 30989646
- Min JJ et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). PubMed: 29682576
- Lee J et al. Enteroendocrine-derived glucagon-like peptide-2 controls intestinal amino acid transport. Mol Metab 6:245-255 (2017). PubMed: 28271031
- Gao H et al. A Low-Protein Diet Enhances Angiotensin II Production in the Lung of Pregnant Rats but not Nonpregnant Rats. J Pregnancy 2016:4293431 (2016). WB . PubMed: 27195150
- Gao H et al. Gestational protein restriction increases angiotensin II production in rat lung. Biol Reprod 88:64 (2013). PubMed: 23365412
- Gao H et al. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod 86:31 (2012). WB, IHC-P ; Rat . PubMed: 22011389
- Gao H et al. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod 86:68 (2012). WB ; Rat . PubMed: 22088913
Images
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Western blot - Anti-ACE2 antibody (ab87436)Anti-ACE2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 115 kDa (possible glycosylated form)
Exposure time: 30 secondsACE2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
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Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436) This image is courtesy of an anonymous Abreview.
ab87436 staining ACE2 in mouse heart tissue sections by immunohistochemistry. Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with ab87436 (1/100 dilution) for 18 hours at 4oC. A HRP-conjugated goat anti-rabbit polyclonal (1/50) was used as the secondary antibody.
