Anti-NBAS antibody (ab242220)
Key features and details
- Rabbit polyclonal to NBAS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NBAS antibody
See all NBAS primary antibodies -
Description
Rabbit polyclonal to NBAS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human NBAS aa 350-400. The exact sequence is proprietary.
Database link: A2RRP1 -
Positive control
- WB: HeLa and Jurkat whole cell lysates. IP: NBAS IP in Jurkat whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to NBAS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NBAS antibody (ab242220)All lanes : Anti-NBAS antibody (ab242220) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 269 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
-
Immunoprecipitation - Anti-NBAS antibody (ab242220)NBAS was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242220 at 3 µl/reaction.
Lysates prepared in NETN buffer.
All lanes : Anti-NBAS antibody (ab242220) at 1/1000 dilution
Lane 1 : ab242220 IP in Jurkat whole cell lysate
Lane 2 : Control IgG IP in Jurkat whole cell lysate
Developed using the ECL technique.
Exposure time: 3 minutes
