Mouse COX2 Matched Antibody Pair Kit (ab220124)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
-
Product name
Mouse COX2 Matched Antibody Pair Kit
See all COX2 / Cyclooxygenase 2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.55 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse COX2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse COX2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210574. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse COX2 Capture Antibody 2 x 50µg 1 x 50µg Mouse COX2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse COX2 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Alternative names
- COX 2
- COX-2
- COX2
see all -
Database links
- Entrez Gene: 19225 Mouse
- SwissProt: Q05769 Mouse
- Unigene: 292547 Mouse
Images
-
Mouse COX2 standard curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Mouse COX2 Matched Antibody Pair Kit (ab220124)To learn more about the advantages of recombinant antibodies see here.
