Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse COX2 ELISA Kit (ab210574)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 97 pg/ml
  • Range: 0.23 ng/ml - 15 ng/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse COX2 ELISA Kit
    See all COX2 / Cyclooxygenase 2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell ext 5 6.9%
    Inter-assay
    Sample n Mean SD CV%
    cell ext 3 9.6%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    97 pg/ml

  • Range

    0.23 ng/ml - 15 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 108 100% - 120%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse COX2 ELISA Kit (ab210574) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of COX2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse COX2 with 97 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Cyclooxygenase-2 (COX2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature mouse COX2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18-55).

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse COX2 ELISA Kit (ab210574)
    Other - Mouse COX2 ELISA Kit (ab210574)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse COX2 standard curve 1X Cell Extraction Buffer PTR.
    Example of mouse COX2 standard curve 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native mouse COX2 in an unstimulated and LPS (1 µg/mL, 6 hr) stimulated Raw 264.7 cell extract based on a 100 and 50 µg/mL extract load, respectively.
    Interpolated concentrations of native mouse COX2 in an unstimulated and LPS (1 µg/mL, 6 hr) stimulated Raw 264.7 cell extract based on a 100 and 50 µg/mL extract load, respectively.

    The concentrations of mouse COX2 were measured in duplicate and interpolated from the COX2 standard curve then corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean COX2 concentration was determined to be 0.203 ng/mL in unstimulated extract and 5.3 ng/mL in LPS stimulated Raw 264.7 extract.

  • Interpolated concentrations of native mouse COX2 in an unstimulated and LPS (1 µg/mL, 6 hr) stimulated Raw 264.7 cell extract based on a 100 µg/mL extract load.
    Interpolated concentrations of native mouse COX2 in an unstimulated and LPS (1 µg/mL, 6 hr) stimulated Raw 264.7 cell extract based on a 100 µg/mL extract load.

    The concentrations of mouse COX2 were measured in duplicate and interpolated from the COX2 standard curve then corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean COX2 concentration was determined to be 0.204 ng/mL in unstimulated extract and 8.7 ng/mL in LPS stimulated Raw 264.7 extract.

  • Sandwich ELISA - Mouse COX2 ELISA Kit (ab210574)
    Sandwich ELISA - Mouse COX2 ELISA Kit (ab210574)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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