Recombinant mouse CD47 protein (Active) (ab231160)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 100 µg/ml.
Images
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Functional Studies - Recombinant mouse CD47 protein (Active) (ab231160)Immobilized Mouse SIRP alpha Mouse IgG2a Fc Tag low endotoxin at 10 µg/mL (100 µL/well) can bind Mouse CD47 with a linear range of 0.078-0.625 µg/mL (QC tested).
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ELISA - Recombinant mouse CD47 protein (Active) (ab231160)Immobilized ab231160 at 10 μg/ml (100 μl/well) can bind Mouse SIRP alpha, Mouse IgG2a Fc Tag with a linear range of 0.156-2.5 μg/ml.
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SDS-PAGE - Recombinant mouse CD47 protein (Active) (ab231160)Mouse CD47 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. -
SDS-PAGE - Recombinant mouse CD47 protein (Active) (ab231160)SDS-PAGE analysis of ab231160 under reducing conditions, stained overnight with Coomassie Blue.
The protein migrates as 30-45 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
