Recombinant rat CD47 protein (Fc Chimera Active) (ab220571)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
Preparation and Storage
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Alternative names
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
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Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Rat CD47 protein (Fc Chimera) (ab220571)
ab220571 on SDS-PAGE under reducing (lane 1) and no-reducing (lane 2) conditions. The gel was stained overnight with Coomassie Blue. As a result of glycosylation, the reduced protein migrates at 50-66 kDa and the non-reduced protein migrates at 100-130 kDa.
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ELISA - Recombinant Rat CD47 protein (Fc Chimera) (ab220571)Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Rat CD47, Fc Tag (ab220571) with a linear range of 2-20 ng/mL.
