APC Anti-CD53 antibody [MRC OX-44] (ab256288)
![APC Anti-CD53 antibody [MRC OX-44] (ab256288)](https://www.abcam.com/ps/products/256/ab256288/Images/ab256288-1-ab256288antiratCD53flowcytometryspleensplenocytes.jpg "APC Anti-CD53 antibody [MRC OX-44] (ab256288)")
Key features and details
- APC Mouse monoclonal [MRC OX-44] to CD53
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-CD53 antibody [MRC OX-44]
See all CD53 primary antibodies -
Description
APC Mouse monoclonal [MRC OX-44] to CD53 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Rat
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Immunogen
Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
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Positive control
- Flow Cyt: Lewis rat splenocytes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-44 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - APC Anti-CD53 antibody [MRC OX-44] (ab256288)
Lewis rat splenocytes stained with ab256288 (right) or mouse IgG1κ Allophycocyanin isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256288) or mouse IgG1κ Allophycocyanin isotype (1x 106 in 100 µl at 1 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on viable lymphocytes.
