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Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44] (ab256285)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44] (ab256285)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [MRC OX-44] to CD53
  • Suitable for: Flow Cyt
  • Reacts with: Rat
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44]
    See all CD53 primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [MRC OX-44] to CD53
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    MRC OX-44
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Signal Transduction
    • Adapters
    • Transmembrane

Images

  • Flow Cytometry - Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44] (ab256285)
    Flow Cytometry - Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44] (ab256285)

    Lewis rat splenocytes stained with ab256285 (right) or mouse IgG1κ Alexa Fluor® 488 isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256285) or mouse IgG1κ Alexa Fluor® 488 isotype (1x 106 in 100 µl at 5 µg/ml (1/100)) for 30 min on ice. The cells were simultaneously stained with CD3.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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