Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17789] to Firefly Luciferase - N-terminal
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Firefly

Product name

Anti-Firefly Luciferase antibody [EPR17789] - N-terminal
See all Firefly Luciferase primary antibodies
Description

Rabbit monoclonal [EPR17789] to Firefly Luciferase - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Firefly
ICC/IF	Firefly
WB	Firefly

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Firefly Luciferase transfected HEK-293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK-293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17789
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185923 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue).
Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Firefly Luciferase cells labeling Firefly Luciferase with purified ab185923 at 1/200 dilution (10ug/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/2000 dilution

Lane 1 : Empty vector (vector control) transfected HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution

Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : MCF-7 (human breast carcinoma) whole cell lysate at 20 µg
Lane 4 : Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg
Lane 5 : SH-SY5Y (human neuroblastoma) whole cell lysate at 20 µg
Lane 6 : Human fetal heart lysate at 10 µg
Lane 7 : Human fetal kidney lysate at 10 µg
Lane 8 : Human fetal spleen lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution

Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Lane 9 : C6 (rat glioma) whole cell lysate lysate
Lane 10 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
Lane 11 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 12 : NIH/3T3 (mouse embryo) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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