Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)
![Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)](https://www.abcam.com/ps/products/232/ab232629/Images/ab232629-321565-anti-firefly-luciferase-antibody-epr17790-flow-cytometry.jpg "Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17790] to Firefly Luciferase - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Firefly
Overview
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Product name
Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free
See all Firefly Luciferase primary antibodies -
Description
Rabbit monoclonal [EPR17790] to Firefly Luciferase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt FireflyICC/IF Firefly
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells.
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General notes
Ab232629 is the carrier-free version of ab185924. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232629 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17790 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)
Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP tagged Firefly Luciferase labeled with purified ab185924 at 1/70 dilution (10µg/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185924).
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![Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)](https://www.abcam.com/ps/products/232/ab232629/Images/ab232629-308732-anti-firefly-luciferase-antibody-epr17790-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185924 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab185924 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185924).
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![Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)](https://www.abcam.com/ps/products/232/ab232629/Images/ab232629-3-benefits-of-recombinant-antibodies.png)
Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free (ab232629)
