Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)
![Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)](https://www.abcam.com/ps/products/252/ab252791/Images/ab252791-365707-antifireflyluciferaseantibodylas126awesternblotfirefly.jpg "Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [LAS126A] to Firefly Luciferase - BSA and Azide free
- Suitable for: WB
- Reacts with: Firefly
Overview
-
Product name
Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free
See all Firefly Luciferase primary antibodies -
Description
Rat monoclonal [LAS126A] to Firefly Luciferase - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Firefly
-
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
-
Positive control
- WB: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate.
-
General notes
ab252791 is the PBS only version of ab241541.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
LAS126A -
Isotype
IgG2b -
Research areas
Images
-
Western blot - Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)All lanes : Anti-Firefly Luciferase antibody [LAS126A] (ab241541) at 1/5000 dilution
Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab241541).
-
![Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)](https://www.abcam.com/ps/products/252/ab252791/Images/ab252791-2-benefits-of-recombinant-antibodies.png)
Anti-Firefly Luciferase antibody [LAS126A] - BSA and Azide free (ab252791)
