Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5
Suitable for: WB
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]
See all Laminin alpha 5/LAMA5 primary antibodies
Description

Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5
Host species

Rabbit
Specificity

This antibody reacts weakly with Laminin alpha 3
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
General notes
This product was previously labelled as Laminin alpha 5

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18919
Isotype

IgG
Research areas

Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1 : Mouse Laminin alpha 5/LAMA5 fragment recombinant protein
Lane 2 : Mouse Laminin alpha 3 fragment recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 404 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.

Mouse Laminin alpha 5/LAMA5 fragment recombinant protein contains aa46-304 with a His-Tag; Mouse Laminin alpha 3 fragment recombinant protein contains aa43-295 with a His-Tag.
Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 404 kDa
Observed band size: 404 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

Lane 1 : Mouse P5 fetal kidney lysate
Lane 2 : Mouse P3 fetal lung lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 404 kDa
Observed band size: 404 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1 and 2: 30 seconds; Lane 3 and 4: 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).

Negative control: mouse liver (PMID:7499364).
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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