Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Laminin alpha 5/LAMA5 antibody [EPR18919]
    See all Laminin alpha 5/LAMA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5

  • Host species

    Rabbit

  • Specificity

    This antibody reacts weakly with Laminin alpha 3

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.

  • General notes

     This product was previously labelled as Laminin alpha 5

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18919

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Mouse Laminin alpha 5/LAMA5 fragment recombinant protein
    Lane 2 : Mouse Laminin alpha 3 fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 404 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.

    Mouse Laminin alpha 5/LAMA5 fragment recombinant protein contains aa46-304 with a His-Tag; Mouse Laminin alpha 3 fragment recombinant protein contains aa43-295 with a His-Tag.

  • Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 404 kDa
    Observed band size: 404 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).

  • Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution

    Lane 1 : Mouse P5 fetal kidney lysate
    Lane 2 : Mouse P3 fetal lung lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 404 kDa
    Observed band size: 404 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 30 seconds; Lane 3 and 4: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).

     Negative control: mouse liver (PMID:7499364).

  • Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)
    Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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