Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)
![Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)](https://www.abcam.com/ps/products/271/ab271947/Images/ab271947-386679-antilamininalpha5antibodyepr18919westernblot.jpg "Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free
See all Laminin alpha 5/LAMA5 primary antibodies -
Description
Rabbit monoclonal [EPR18919] to Laminin alpha 5/LAMA5 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody reacts weakly with Laminin alpha 3
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Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse Laminin alpha 5/LAMA5 and alpha 3 fragment recombinant proteins; Human fetal kidney lysate; A549 whole cell lysate; Mouse P5 fetal kidney and P3 fetal lung lysates; Rat kidney lysate.
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General notes
This product was previously labelled as Laminin alpha 5.
ab271947 is the carrier-free version of ab184330. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18919 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)All lanes : Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] (ab184330) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 400 kDa
Observed band size: 404 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:12559092; PMID:9624186).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, sodium azide and BSA (ab184330).
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![Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)](https://www.abcam.com/ps/products/271/ab271947/Images/ab271947-3-benefits-of-recombinant-antibodies.png)
Anti-Laminin alpha 5/LAMA5 antibody [EPR18919] - BSA and Azide free (ab271947)
