Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11767(B)] to IFI16 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free
    See all IFI16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11767(B)] to IFI16 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249529 is the carrier-free version of ab169788. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Western blot - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    All lanes : Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

    Lane 1 : U937 treated with IFN gamma cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa



    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunocytochemistry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

    Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.
  • Immunocytochemistry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunocytochemistry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The image shows nuclear staining on Jurkat cells.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

    ab169788 showing -ve staining in Human gastric adenocarcinoma tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

    This data was developed using ab169788, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    This data was developed using ab169788, the same antibody clone in a different buffer formulation.ab169788 showing +ve staining in Human normal spleen tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    This data was developed using ab169788, the same antibody clone in a different buffer formulation.ab169788 showing -ve staining in Human normal uterus tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    This data was developed using ab169788, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    This data was developed using ab169788, the same antibody clone in a different buffer formulation.Flow cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
    Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.