Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)
![Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)](https://www.abcam.com/ps/products/200/ab200632/Images/ab200632-280885-anti-human-kappa-chain-antibody-epr11633-alexa-fluor-488-immunohistochemistry.jpg "Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR11633] to Human Kappa Chain
- Suitable for: IHC-Fr
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633]
See all Human Kappa Chain primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR11633] to Human Kappa Chain -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Human Kappa Chain. The exact sequence is proprietary.
Database link: P01834 -
Positive control
- IHC-Fr: normal human tonsil tissue sections
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11633 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)
IHC image of human Kappa Chain staining in a section of frozen normal human tonsil*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab200632 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)](https://www.abcam.com/ps/products/200/ab200632/Images/ab200632-280884-anti-human-kappa-chain-antibody-epr11633-alexa-fluor-488-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)IHC image of human Kappa Chain staining in a section of frozen normal human cerebral cortex*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab200632 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)](https://www.abcam.com/ps/products/200/ab200632/Images/ab200632-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Human Kappa Chain antibody [EPR11633] (ab200632)
