Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)
![Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)](https://www.abcam.com/ps/products/238/ab238618/Images/ab238618-339416-ab2386183.jpg "Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)")
Key features and details
- Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ICC, ELISA
Overview
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Product name
Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)
See all Kappa light chain secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
ab238618 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The Fc region of ab238618 has been engineered to eliminate Fc receptor binding.
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Tested applications
Suitable for: WB, ICC, ELISAmore details -
Immunogen
Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM103 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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ELISA - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)
A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Western blot - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)](https://www.abcam.com/ps/products/238/ab238618/Images/ab238618-339415-ab238618WB.jpg)
Western blot - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)All lanes : Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618) at 0.2 µg/ml
Lanes 1 & 3 : Non-reduced(-) mouse IgG1?
Lanes 2 & 4 : Reduced(+) mouse IgG1?
Predicted band size: 25 kDaThis antibody reacts to non-reduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
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![ELISA - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)](https://www.abcam.com/ps/products/238/ab238618/Images/ab238618-324209-ab238618.jpg)
ELISA - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618)ELISA of mouse immunoglobulins shows ab238618 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/ml, 50 ng/ml, or 10 ng/ml of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.