Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB)
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-beta glucuronidase (GUSB) antibody [EPR10616]
See all beta glucuronidase (GUSB) primary antibodies
Description

Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in Human beta glucuronidase (GUSB) (UniProt ID: P08236).
Positive control
- Human fetal liver lysate, K562, HepG2 and 293T cell lysates, paraffin-embedded Human liver and skin tissue, HepG2 cells, K562 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10616
Isotype

IgG
Research areas

Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

All lanes : Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution

Predicted band size: 78 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Immunofluorescent analysis of HepG2 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution.
Flow Cytometry - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Flow cytometric analysis of permeabilized K562 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution (red) or a rabbit IgG negative (green).
Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com