Recombinant human EGFR (mutated T790M + L858R) protein (Active) (ab271495)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human EGFR (mutated T790M + L858R) protein (Active)
See all EGFR proteins and peptides -
Biological activity
Specific Activity: 1200 pmol/min/μg.Assay was performed in kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 30 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent. -
Purity
>= 50 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKV LGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMA SVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNW CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEK EYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDG IPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIE FSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYL IPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQ RYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNP APSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISL DNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA -
Predicted molecular weight
88 kDa -
Amino acids
668 to 1210 -
Modifications
mutated T790M + L858R -
Tags
His tag C-Terminus , GST tag N-Terminus -
Additional sequence information
Cytoplasmic domain.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 0.79% Tris HCl, 1.45% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human EGFR (mutated T790M + L858R) protein (Active) (ab271495)Specific activity of ab271495.
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SDS-PAGE - Recombinant human EGFR (mutated T790M + L858R) protein (Active) (ab271495)SDS-PAGE analysis of 0.2 µg ab271495.