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Cell Biology Other Antibodies

Anti-TMUB2 antibody [EPR13741] (ab181183)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-TMUB2 antibody [EPR13741] (ab181183)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13741] to TMUB2
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-TMUB2 antibody [EPR13741]
    See all TMUB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13741] to TMUB2
  • Host species

    Rabbit
  • Specificity

    Based on the sequence analysis, ab181183 recognizes 4 isoforms with predicted MW of 34KDa, 32KDa, 19KDa and 10KDa, respectively.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HEK293, HepG2 and A431 cell lysates. HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13741
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Western blot - Anti-TMUB2 antibody [EPR13741] (ab181183)
    Western blot - Anti-TMUB2 antibody [EPR13741] (ab181183)
    All lanes : Anti-TMUB2 antibody [EPR13741] (ab181183) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 10-34 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-TMUB2 antibody [EPR13741] (ab181183)
    Flow Cytometry - Anti-TMUB2 antibody [EPR13741] (ab181183)

    Flow cytometry analysis of TMUB2 expression in HeLa cells using ab181183 at 1/150 dilution (red) and a rabbit IgG as negative control (green).

  • Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] (ab181183)
    Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] (ab181183)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-TMUB2 antibody [EPR13741] (ab181183)
    Anti-TMUB2 antibody [EPR13741] (ab181183)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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