Anti-C20orf77 antibody (ab137246)
Key features and details
- Rabbit polyclonal to C20orf77
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-C20orf77 antibody
See all C20orf77 primary antibodies -
Description
Rabbit polyclonal to C20orf77 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 276-326 of Human C20orf77 (NP_067038.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899), mouse NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab137246 was affinity purified using an epitope specific to C20orf77 immobilized on solid support . -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C20orf77 antibody (ab137246)All lanes : Anti-C20orf77 antibody (ab137246) at 0.1 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : 293T whole cell
lysate at 50 µg
Lane 4 : Jurkat whole cell
lysate at 50 µg
Lane 5 : mouse NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-C20orf77 antibody (ab137246)Immunoprecipitation with ab137246 at 6µg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Western blot labelling C20orf77 with ab372461 at 1 µg/ml. Detected by chemiluminescence with exposure time of 10 seconds.
