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Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PHOX2B antibody [EPR14423] - C-terminal
    See all PHOX2B primary antibodies

  • Description

    Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Neuro-2a and SH-SY5Y cell lysate. IHC-P: Human neuroblastoma tissue. ICC/IF: HeLa and SH-SY5Y cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B  with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    Western blot - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    All lanes : Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) at 1/1000 dilution

    Lane 1 : Neuro-2a cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B  with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    Immunocytochemistry/ Immunofluorescence - Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
    Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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