Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MUC13 antibody [EPR21901] (ab235450)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21901] to MUC13
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-MUC13 antibody [EPR21901]
See all MUC13 primary antibodies
Description

Rabbit monoclonal [EPR21901] to MUC13
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HT-29, SW480, HCT 116 and LoVo whole cell lysates. IHC-P: Human colon, colorectal cancer and gastric cancer tissue. ICC/IF: LoVo and HT-29 cells. Flow Cyt: LoVo cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21901
Isotype

IgG
Research areas

Western blot - Anti-MUC13 antibody [EPR21901] (ab235450)

All lanes : Anti-MUC13 antibody [EPR21901] (ab235450) at 1/1000 dilution

Lane 1 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : Human ovary cancer lysate at 10 µg
Lane 5 : LoVo (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 55 kDa
Observed band size: 120,35,80 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-3 and 5: 3 minutes, Lane 4: 26 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 15904467). Lane 3 was developed using a high sensitivity ECL substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in epithelial cells of the normal human colon (PMID: 17360025) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-MUC13 antibody [EPR21901] (ab235450)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/40 (red) compared with a Isotype control details (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human gastric cancer (PMID: 15904467) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the apical surface and in the cytoplasm in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the LoVo cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-MUC13 antibody [EPR21901] (ab235450)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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