Anti-MUC13 antibody (ab65109)
Key features and details
- Rabbit polyclonal to MUC13
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MUC13 antibody
See all MUC13 primary antibodies -
Description
Rabbit polyclonal to MUC13 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) from an internal sequence.
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Positive control
- WB: Extracts from COLO, 293 and HeLa cells. IHC-P: Human colon carcinoma tissue. IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MUC13 antibody (ab65109)All lanes : Anti-MUC13 antibody (ab65109) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from HeLa cells
Lane 4 : extracts from HeLa cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody (ab65109) -
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody (ab65109)ICC/IF image of ab65109 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
