Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21901] to MUC13 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MUC13 antibody [EPR21901] - BSA and Azide free
    See all MUC13 primary antibodies

  • Description

    Rabbit monoclonal [EPR21901] to MUC13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human gastric cancer tissue.

  • General notes

    ab237590 is the carrier-free version of ab235450.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Flow Cytometry - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Flow Cytometry - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/40 (red) compared with a Isotype control details (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the apical surface and in the cytoplasm in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in epithelial cells of the normal human colon (PMID: 17360025) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the LoVo cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human gastric cancer (PMID: 15904467) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235450).

     

  • Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)
    Anti-MUC13 antibody [EPR21901] - BSA and Azide free (ab237590)

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