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Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [C14] to MUC13 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MUC13 antibody [C14] - BSA and Azide free
    See all MUC13 primary antibodies

  • Description

    Mouse monoclonal [C14] to MUC13 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: LoVo cells. Flow Cyt: T84 cells.

  • General notes

    ab255736 is the PBS only version of ab231159.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)
    Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab231159).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling MUC13 with ab1231159 at 10.82µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LoVo cell line. The nuclear counterstain is DAPI (blue).
    Counterstains:
    ab179504  Anti-beta IV Tubulin antibody - Microtubule Marker
    ab150080 AlexaFluor®594 Goat anti-Rabbit secondary.

    The negative controls are as follows:

    -ve control 1: ab231159 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.

    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.

  • Flow Cytometry - Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)
    Flow Cytometry - Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab231159).

    Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized T84 (human colon epithelial cell) cells labeling MUC13 with ab231159 at a 1.082µg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

    Gated on viable cells.

  • Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)
    Anti-MUC13 antibody [C14] - BSA and Azide free (ab255736)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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