Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6731(2)] to LMO4 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free
    See all LMO4 primary antibodies

  • Description

    Rabbit monoclonal [EPR6731(2)] to LMO4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248352 is the carrier-free version of ab131030 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248352 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Actual band size : 17 kDa.

  • OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    OI-RD Scanning - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    This data was developed using ab131030, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    Western blot - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Mouse cerebral cortex tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using ab131030, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    Immunoprecipitation - Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    This data was developed using ab131030, the same antibody clone in a different buffer formulation.Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2). Lane 1 (input): Jurkat whole cell lysate 10ug Lane 2 (+): ab131030 + Jurkat whole cell lysate. Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131030 in Jurkat whole cell lysate VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking/Diluting buffer 5% NFDM/TBST.
  • Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)
    Anti-LMO4 antibody [EPR6731(2)] - BSA and Azide free (ab248352)

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