Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)
Key features and details
- Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS
- Suitable for: IP, WB, ICC, ICC/IF, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody
See all Glutamyl Prolyl tRNA synthetase/PARS primary antibodies -
Description
Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS -
Host species
Rabbit -
Specificity
This antibody reacts specifically with EPRS protein. -
Tested applications
Suitable for: IP, WB, ICC, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531) at 1/10000 dilution + HEK293 cell line at 30 µg
Predicted band size: 163 kDa
Observed band size: 163 kDa
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Immunocytochemistry - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Ab31531 staining Glutamyl Prolyl tRNA synthetase/PARS in Hela cell line by Immunofluorescence.
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Western blot - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531) at 1/20000 dilution + whole cell lysate prepared from HEK-293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 163 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531)IHC image of ab31531 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31531, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody (ab31531) This image is courtesy of an anonymous Abreviewab31531 staining Glutamyl Prolyl tRNA synthetase/PARS in the Human Liver cell line Huh7 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% and blocked with 10% Serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 10% Serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
