Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498)
Key features and details
- Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal -
Description
Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase/PARS - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment within Human Glutamyl Prolyl tRNA synthetase/PARS (C terminal). The exact sequence is proprietary.
Database link: P07814 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse intestine tissue; Rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498)All lanes : Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 171 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498)Paraffin-embedded mouse intestine tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498)Paraffin-embedded rat brain tissue stained for Glutamyl Prolyl tRNA synthetase/PARS using ab229498 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Glutamyl Prolyl tRNA synthetase/PARS antibody - C-terminal (ab229498)Glutamyl Prolyl tRNA synthetase/PARS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229498. Western blot was performed from the immunoprecipitate using ab229498. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229498 in HEK-293T whole cell extract.
Lane 2: ab229498 IP in HEK-293T whole cell extract.
