Antibodies, Proteins, Kits and Reagents for Life Science

Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR086G] to beta Catenin - BSA free
Suitable for: IP, ICC, IHC-P
Reacts with: Human

Product name

Anti-beta Catenin antibody [BLR086G] - BSA free
See all beta Catenin primary antibodies
Description

Rabbit monoclonal [BLR086G] to beta Catenin - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human beta Catenin aa 725-775. The exact sequence is proprietary. NP_001895.1
Database link: P35222
Positive control
- IHC-P: Human colon carcinoma tissue. Human plasmacytoma. ICC: OVCAR-3 cells. IP: HeLa and HepG2 whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272069 is the carrier-free version of ab265591. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR086G
Isotype

IgG
Research areas

Immunocytochemistry - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 (Human ovarian carcinoma cell line) cells labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265591).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human plasmacytoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265591).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265591).
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

beta Catenin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265591).
Immunoprecipitation - Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

beta Catenin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
Cells prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265591).
Anti-beta Catenin antibody [BLR086G] - BSA free (ab272069)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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