Anti-CTNNA1 antibody (ab227181)
Key features and details
- Rabbit polyclonal to CTNNA1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CTNNA1 antibody
See all CTNNA1 primary antibodies -
Description
Rabbit polyclonal to CTNNA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human CTNNA1 (internal sequence). The exact sequence is proprietary.
Database link: P35221 -
Positive control
- WB: HepG2, NIH/3T3, JC and PC-12 whole cell lysates. IHC-P: Mouse prostate and intestine tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (ab227181)
Paraffin-embedded mouse intestine tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-CTNNA1 antibody (ab227181)All lanes : Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 100 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (ab227181)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using ab227181 at 1/500 dilution in ICC/IF.
Nuclear Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-CTNNA1 antibody (ab227181)All lanes : Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : JC whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-CTNNA1 antibody (ab227181)Anti-CTNNA1 antibody (ab227181) at 1/10000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-CTNNA1 antibody (ab227181)Anti-CTNNA1 antibody (ab227181) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 100 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody (ab227181)Paraffin-embedded mouse prostate carcinoma tissue stained for CTNNA1 using ab227181 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody (ab227181)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CTNNA1 (green) using ab227181 at 1/500 dilution in ICC/IF.
Counterstain: alpha Tubulin was stained by an alpha Tubulin antibody at 1/500 dilution (red). Nuclear Counterstain: Hoechst 33342 (blue).
