Anti-CTNNA1 antibody [1G5] (ab231306)
![Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-324645-anti-alpha-1-catenin-antibody-1g5-immunofluorescence.jpg "Anti-CTNNA1 antibody [1G5] (ab231306)")
Key features and details
- Mouse monoclonal [1G5] to CTNNA1
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CTNNA1 antibody [1G5]
See all CTNNA1 primary antibodies -
Description
Mouse monoclonal [1G5] to CTNNA1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human CTNNA1. (A recombinant maltose binding protein fused to full length human alpha catenin).
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Positive control
- ICC/IF: A431 cells WB: HAP1, rat colon
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General notes
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product was previously labelled as alpha 1 Catenin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1G5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CTNNA1 antibody [1G5] (ab231306)
ab231306 staining CTNNA1 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231306 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)](https://www.abcam.com/ps/products/231/ab231306/Images/ab231306-326819-anti-alpha-1-catenin-antibody-1g5-western-blot.jpg)
Western blot - Anti-CTNNA1 antibody [1G5] (ab231306)All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CTNNA1 KO whole cell lysate
Lane 3 : Rat colon whole tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 100 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab231306).
ab231306 was shown to specifically react with CTNNA1 (α-catenin) in wild type HAP1 cells. No band was observed when CTNNA1 (α-catenin) knockout samples were used. Wild-type and CTNNA1 (α-catenin) knockout samples were subjected to SDS-PAGE. ab231306 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentraion and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
