Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1793Y] to CTNNA1 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free
    See all CTNNA1 primary antibodies

  • Description

    Rabbit monoclonal [EP1793Y] to CTNNA1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse heart and kidney lysate. Rat brain and kidney lysate. HeLa, A431 and HUVEC whole cell lysate. IHC-P: Rat and human stomach tissue and mouse liver tissue IP: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

  • General notes

    ab226010 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product was previously labelled as alpha 1 Catenin

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Immunoprecipitation - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    ab190685 at 1/100 dilution immunoprecipitating CTNNA1 in Jurkat HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51032 in HeLa whole cell lysate
    For western blotting, ab51032 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded rat stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on rat stomach.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded mouse liver tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on mouse liver.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    Paraffin-embedded human stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.

    Positive staining was seen on human stomach.

    The section was incubated with ab51032 for 30 minutes at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    All lanes : Anti-CTNNA1 antibody [EP1793Y] (ab51032) at 1/10000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 7 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 100 kDa
    Observed band size: 100 kDa



    Expsure times

    Lane 1-4: 180 seconds

    Lane 5,7: 40 seconds

    Lane 6: 5 seconds

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51032).

  • Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Western blot - Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

    This WB data was generated using the same anti-CTNNA1 antibody clone [EP1793Y] in a different buffer formulation (cat# ab51032).

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: CTNNA1 HAP1 whole cell lysate (20 µg) 
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51032 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Ab51032 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)
    Anti-CTNNA1 antibody [EP1793Y] - Low endotoxin, Azide free (ab226010)

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