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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class V Lamins

Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)

Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9700(B)] to Lamin B2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free
    See all Lamin B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR9700(B)] to Lamin B2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248858 is the carrier-free version of ab138516. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248858 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9700(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Lamins
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleoplasm
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Lamins

Images

  • Western blot - Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
    Western blot - Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
    All lanes : Anti-Lamin B2 antibody [EPR9700(B)] (ab138516) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab138516, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
    Flow Cytometry - Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
    This data was developed using ab138516, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized HeLa cells with ab138516 at a dilution of 1/10 (red) or a rabbit IgG negative (green).
  • Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)
    Anti-Lamin B2 antibody [EPR9700(B)] - BSA and Azide free (ab248858)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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