Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Emerin antibody (ab40725)

Key features and details

  • Rabbit polyclonal to Emerin
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Emerin antibody
    See all Emerin primary antibodies

  • Description

    Rabbit polyclonal to Emerin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Emerin aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab40724)

  • Positive control

    • ab40725 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) ab40725 gave a positive result in the following nuclear lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HepG2 (Human hepatocellular liver carcinoma cell line)

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25, 29 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25, 29 kDa).

Target

  • Function

    Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

  • Tissue specificity

    Skeletal muscle, heart, colon, testis, ovary and pancreas.

  • Involvement in disease

    Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.

  • Sequence similarities

    Contains 1 LEM domain.

  • Post-translational
    modifications

    Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.

  • Cellular localization

    Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EDMD antibody
    • Emd antibody
    • EMD_HUMAN antibody
    • Emerin antibody
    • Emery Dreifuss muscular dystrophy antibody
    • STA antibody
    see all

Images

  • Western blot - Anti-Emerin antibody (ab40725)
    Western blot - Anti-Emerin antibody (ab40725)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Emerin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab40725 observed at 35 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab40725 was shown to specifically react with Emerin in wild-type HAP1 cells as signal was lost in Emerin knockout cells. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. Ab40725 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Emerin antibody (ab40725)
    Western blot - Anti-Emerin antibody (ab40725)
    All lanes : Anti-Emerin antibody (ab40725) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : Jurkat nuclear extract lysate (ab14844)
    Lane 5 : Hep G2 whole cell lysate (ab7900)
    Lane 6 : Hep G2 nuclear extract lysate (ab14660)
    Lane 7 : A-431 whole cell lysate (ab7909)
    Lane 8 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 25, 29 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (ab40725)
    Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (ab40725)
    ICC/IF image of ab40725 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40725, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References (1)

Publishing research using ab40725? Please let us know so that we can cite the reference in this datasheet.

ab40725 has been referenced in 1 publication.

  • Khanna R  et al. E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins. FEBS J 285:2243-2262 (2018). PubMed: 29676528

Images

  • Western blot - Anti-Emerin antibody (ab40725)
    Western blot - Anti-Emerin antibody (ab40725)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Emerin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab40725 observed at 35 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab40725 was shown to specifically react with Emerin in wild-type HAP1 cells as signal was lost in Emerin knockout cells. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. Ab40725 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Emerin antibody (ab40725)
    Western blot - Anti-Emerin antibody (ab40725)
    All lanes : Anti-Emerin antibody (ab40725) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : Jurkat nuclear extract lysate (ab14844)
    Lane 5 : Hep G2 whole cell lysate (ab7900)
    Lane 6 : Hep G2 nuclear extract lysate (ab14660)
    Lane 7 : A-431 whole cell lysate (ab7909)
    Lane 8 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 25, 29 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (ab40725)
    Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (ab40725)
    ICC/IF image of ab40725 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40725, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

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