Anti-ZMPSTE24 antibody (ab234832)
Key features and details
- Rabbit polyclonal to ZMPSTE24
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZMPSTE24 antibody -
Description
Rabbit polyclonal to ZMPSTE24 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human ZMPSTE24 aa 200-350.
Database link: O75844 -
Positive control
- IHC-P: Human liver and gastric cancer tissue. ICC/IF: PC-3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
PurityClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab234832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P 1/20 - 1/200. ICC/IF 1/50 - 1/200. Target
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Function
Proteolytically removes the C-terminal three residues of farnesylated proteins. Acts on lamin A/C. -
Tissue specificity
Widely expressed. High levels in kidney, prostate, testis and ovary. -
Involvement in disease
Defects in ZMPSTE24 are the cause of mandibuloacral dysplasia with type B lipodystrophy (MADB) [MIM:608612]. Mandibuloacral dysplasia (MAD) is a rare autosomal recessive disorder characterized by mandibular and clavicular hypoplasia, acroosteolysis, delayed closure of the cranial suture, joint contractures, and types A or B patterns of lipodystrophy. Type B lipodystrophy observed in MADB, is characterized by generalized fat loss.
Defects in ZMPSTE24 are a cause of lethal tight skin contracture syndrome (LTSCS) [MIM:275210]; also called restrictive dermopathy (RD). Lethal tight skin contracture syndrome is a rare disorder mainly characterized by intrauterine growth retardation, tight and rigid skin with erosions, prominent superficial vasculature and epidermal hyperkeratosis, facial features (small mouth, small pinched nose and micrognathia), sparse/absent eyelashes and eyebrows, mineralization defects of the skull, thin dysplastic clavicles, pulmonary hypoplasia, multiple joint contractures and an early neonatal lethal course. Liveborn children usually die within the first week of life. The overall prevalence of consanguineous cases suggested an autosomal recessive inheritance. -
Sequence similarities
Belongs to the peptidase M48A family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 10269 Human
- Entrez Gene: 230709 Mouse
- Omim: 606480 Human
- SwissProt: O75844 Human
- SwissProt: Q80W54 Mouse
- Unigene: 132642 Human
- Unigene: 721062 Human
- Unigene: 34399 Mouse
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Alternative names
- CAAX prenyl protease 1 homolog antibody
- FACE-1 antibody
- FACE1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)
Paraffin-embedded human gastric cancer tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZMPSTE24 antibody (ab234832)PC-3 (human prostate adenocarcinoma cell line) cells labeling ZMPSTE24 using ab234832 at 1/100 dilution in ICC/IF. Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)Paraffin-embedded human liver tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab234832 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)
Paraffin-embedded human gastric cancer tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ZMPSTE24 antibody (ab234832)
PC-3 (human prostate adenocarcinoma cell line) cells labeling ZMPSTE24 using ab234832 at 1/100 dilution in ICC/IF. Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)
Paraffin-embedded human liver tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.