Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9701(B)] to Lamin B2 - BSA and Azide free
  • Suitable for: IP, ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free
    See all Lamin B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9701(B)] to Lamin B2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HepG2 cell lysate; IHC: Human bladder cancer tissue; ICC: HepG2 cells; WB: HepG2 and HeLa cells, Mouse and rat testis and rat spleen lysates. Flow cyt: HepG2 cells.

  • General notes

    The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat.

    Ab240124 is the carrier-free version of ab151735. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240124 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    All lanes : Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse testis lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab151735, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Western blot - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    This data was developed using ab151735, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Immunocytochemistry - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

    This data was developed using ab151735, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Flow Cytometry - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

    This data was developed using ab151735, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab240124 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

    This data was developed using ab151735, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab240124 at 1:100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

    This data was developed using ab151735, the same antibody clone in a different buffer formulation.

    Purified ab151735 at 1/20 dilution (0.5µg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab151735 + HepG2 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 68 kDa

  • Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)
    Anti-Lamin B2 antibody [EPR9701(B)] - BSA and Azide free (ab240124)

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