Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9701(B)] to Lamin B2
Suitable for: ICC, Flow Cyt, WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Lamin B2 antibody [EPR9701(B)]
See all Lamin B2 primary antibodies
Description

Rabbit monoclonal [EPR9701(B)] to Lamin B2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Lamin B2 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q03252
Positive control
- IP: HepG2 cell lysate; IHC: Human bladder cancer tissue; ICC: HepG2 cells; WB: HepG2 and HeLa cells, Mouse and rat testis and rat spleen lysates. Flow cyt: HepG2 cells.
General notes
The human and rat recommendation is based on the WB results. We do not guarantee IHC-P for human and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9701(B)
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Lamins
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Lamins

Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

All lanes : Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse testis lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Blocking Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Anti-Lamin B2 antibody [EPR9701(B)] (ab151735) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Blocking Buffer and concentration: 5% NFDM/TBST
Immunocytochemistry - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1:50 dilution (2.18 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Lamin B2 with Purified ab151735 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder cancer tissue sections labeling Lamin B2 with Purified ab151735 at 1:100 dilution (1.09 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

Purified ab151735 at 1/20 dilution (0.5µg) immunoprecipitating Lamin B2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab151735 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151735 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 68 kDa
Anti-Lamin B2 antibody [EPR9701(B)] (ab151735)

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