Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16537] to TPP1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TPP1 antibody [EPR16537] - BSA and Azide free
    See all TPP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16537] to TPP1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.

  • General notes

    Ab209692 is the carrier-free version of ab195234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab209692 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    This data was developed using ab195234, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
    (inset: negative control). Note: Cytoplasm staining on human placenta tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    Western blot - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    This data was developed using ab195234, the same antibody clone in a different buffer formulation.

    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

  • Western blot - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    Western blot - Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    This data was developed using ab195234, the same antibody clone in a different buffer formulation.

    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

  • Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)
    Anti-TPP1 antibody [EPR16537] - BSA and Azide free (ab209692)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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