Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-POLD1 antibody [EPR15118] (ab186407)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15118] to POLD1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-POLD1 antibody [EPR15118]
See all POLD1 primary antibodies
Description

Rabbit monoclonal [EPR15118] to POLD1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- MOLT4, HeLa, Jurkat, K562, C6, Raw264.7 and PC12 cell lysates; Human cervix carcinoma and colon tissues; HeLa and K562 cells.
General notes
This product was previously labelled as DNA Polymerase delta

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15118
Isotype

IgG
Research areas

Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)

All lanes : Anti-POLD1 antibody [EPR15118] (ab186407) at 1/10000 dilution

Lane 1 : MOLT4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

ab186407 staining POLD1, catalytic subunit in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-POLD1 antibody [EPR15118] (ab186407)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling POLD1, catalytic subunit (red) with purified ab186407 at a dilution of 1/250. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.
Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)

All lanes : Anti-POLD1 antibody [EPR15118] (ab186407) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

Immunofluorescent analysis of acetone-fixed K562 cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Anti-POLD1 antibody [EPR15118] (ab186407)

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