Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15118] to POLD1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-POLD1 antibody [EPR15118] - BSA and Azide free
    See all POLD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15118] to POLD1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240300 is the carrier-free version of ab186407 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DNA Polymerase delta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Flow Cytometry - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling POLD1, catalytic subunit (red) with purified ab186407 at a dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    ab186407 staining POLD1, catalytic subunit in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

    Immunofluorescent analysis of acetone-fixed K562 cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186407).

  • Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)
    Anti-POLD1 antibody [EPR15118] - BSA and Azide free (ab240300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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