Anti-POLD1 antibody (ab264345)
Key features and details
- Rabbit polyclonal to POLD1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-POLD1 antibody
See all POLD1 primary antibodies -
Description
Rabbit polyclonal to POLD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Hamster, Cow
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Immunogen
Synthetic peptide within Human POLD1 aa 930-980. The exact sequence is proprietary. NP_002682.2
Database link: P28340 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK1 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLD1 antibody (ab264345)All lanes : Anti-POLD1 antibody (ab264345) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-POLD1 antibody (ab264345)POLD1 was immunoprecipitated from Jurkat whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264345 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264345 at 1 μg/ml.
Lane 1: ab264345 IP in Jurkay whole cell lysate.
Lane 2: ab264345 IP in Jurkay whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Different batches used for IP in lanes 1 and 2.
